Triacylglyceride Composition of Cottonseed Oil by HPLC and GC

The triacylglyceride components of cottonseed oil were isolated and positively identified by a combination of high performance liquid chromatography (HPLC) and gas chromatography (GC). Both reversed-phase HPLC and capillary GC were capable of separating the oil into triacylglyceride peaks. These peaks were isolated by HPLC and their component acyl groups were converted to fatty acid methyl ester derivatives. The acyl constituents for each triacylglyceride were determined by GC analysis, thus positively identifying the triacylglyceride associated with each HPLC peak. The triacylglyceride elution order agreed with predictive methods. HPLC and capillary GC peaks were correlated by peak area, thus identifying the GC peaks. The corresponding GC elution order of triacylglycerides also agreed with predictive methods.